thymine nucleotides การใช้

• Since thymine nucleotides are precursors of DNA ( but not RNA ), the prefix " deoxy " is often left out, i . e ., deoxythymidine is often just called thymidine.
• This reaction serves two cellular purposes : providing a precursor ( dUMP ) for the synthesis of thymine nucleotides needed for DNA replication, and limiting intracellular pools of dUTP . Elevated levels of dUTP lead to increased incorporation of uracil into DNA, which induces extensive excision repair mediated by uracil glycosylase.
• 3'RACE-PCR uses the natural polyA tail that exists at the 3'end of all eukaryotic mRNAs for priming during reverse transcription, so this method does not require the addition of nucleotides by TdT . cDNAs are generated using an Oligo-dT-adaptor primer ( a primer with a short sequence of deoxy-thymine nucleotides ) that complements the polyA stretch and adds a special adaptor sequence to the 5'end of each cDNA . PCR is then used to amplify 3'cDNA from a known region using a sense GSP, and an anti-sense primer complementary to the adaptor sequence.